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Biofire FilmArray Panel

  • March 15,2022
  • 1 Min Read
Biofire FilmArray Panel

The Biofire FilmArray Panels perform multiplex testing to detect pathogens on specifically mentioned samples:

Panel

Sample Type

Container

Transportation Temperature

Turnaround Time

BioFire Blood Culture Identification 2 (BCID2) FilmArray Panel

Blood, pus or body fluid

Blood culture bottle (paired)

Room temperature

4 hours

(post blood culture flagging positive)

Gastrointestinal FilmArray Panel

Stool

Cary Blair transport medium/ sterile container

2°C - 8 °C (if transported in Cary Blair medium)

Frozen (if Cary Blair medium is not available)
 

4 hours

Respiratory 2.1 FilmArray Panel

Nasopharyngeal swab

3ml of viral transport media (VTM) / saline

At room temperature for up to 4 hours (15°C - 25 °C)

Refrigerated for up to 3 days (2°C - 8 °C)

4 hours

Pneumonia Plus FilmArray Panel

Bronchoalveolar lavage (BAL)

sputum (induced and expectorated) 

endotracheal aspirate (ETA)

Sterile container

Refrigerated for up to 1 day (2°C - 8°C)

4 hours

Meningitis/Encephalitis (ME) FilmArray Panel

CSF

Sterile container

CSF in frozen condition or transported on ice within 24-30 hrs

4 hours

Quality Control: In built extraction & PCR control in the lyophilized form in the pouches ensures the quality check at critical steps of extraction & PCR for sample inhibitory effect. 

Result terminology:

  • Detected: 

When the result is shown as detected for a specific pathogen/ resistance gene, it means:

  • Controls were successfully passed, AND

  • The specific genetic material for the pathogen/ resistance gene was detected.

  • Not Detected:

When the result is shown as not detected for a specific pathogen/ resistance gene, it means:

  • Controls were successfully passed, AND

  • The specific genetic material for the pathogen/ resistance gene was not detected.

 

Interpretation of antimicrobial resistance genes in the pneumonia and BCID2 Biofire panels:

Antimicrobial resistance gene

Interpretation

CTX-M

Production of extended-spectrum β-lactamase (ESBL)(1)

Resistance to a wide range of commonly used β-lactam antibiotics including third generation cephalosporins (cefotaxime and ceftriaxone, but not generally against ceftazidime)

IMP

Carbapenemase production(2)

Resistance to carbapenems

KPC 

Carbapenemase production(3)

Resistance to carbapenems

MCR-1

Colistin resistance(4)

MecA / MecC, MREJ

Presence of MRSA(5)

NDM

Carbapenemase production(3)

Resistance to carbapenems

OXA-48

Carbapenemase production(3)

Resistance to carbapenems

VanA/B

Vancomycin resistance(6)

VIM

Carbapenemase production(3)

Resistance to carbapenems

 

References:

1.     Rossolini GM, D’Andrea MM, Mugnaioli C. The spread of CTX-M-type extended-spectrum β-lactamases. Clin Microbiol Infect. 2008 Jan;14:33–41. 

2.     Lowe CF, Matic N, Champagne S, Romney MG, Leung V, Ritchie G. The Brief Case: IMP, the Uncommonly Common Carbapenemase. Burnham C-AD, editor. J Clin Microbiol [Internet]. 2020 Mar 25 [cited 2021 Dec 21];58(4). Available from: https://journals.asm.org/doi/10.1128/JCM.01094-19

3.     Han R, Shi Q, Wu S, Yin D, Peng M, Dong D, et al. Dissemination of Carbapenemases (KPC, NDM, OXA-48, IMP, and VIM) Among Carbapenem-Resistant Enterobacteriaceae Isolated From Adult and Children Patients in China. Front Cell Infect Microbiol. 2020 Jul 3;10:314. 

4.     Li B, Yin F, Zhao X, Guo Y, Wang W, Wang P, et al. Colistin Resistance Gene mcr-1 Mediates Cell Permeability and Resistance to Hydrophobic Antibiotics. Front Microbiol. 2020 Jan 10;10:3015. 

5.     Becker K, Denis O, Roisin S, Mellmann A, Idelevich EA, Knaack D, et al. Detection of mecA and mecC Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay. Burnham C-AD, editor. J Clin Microbiol. 2016 Jan;54(1):180–4. 

6.     Phukan C, Lahkar M, Ranotkar S, Saikia K. Emergence of vanA gene among vancomycin-resistant enterococci in a tertiary care hospital of North - East India. Indian J Med Res. 2016;143(3):357. 

 

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