Suburban blog

Detect Antibodies Presence using Antinuclear Antibodies Technique By IFA

I. The Gold Standard technique to detect presence of antibodies

II. Combination of HEp-2010 cells and primate liver cells provides

  • Pattern differentiation which corresponds to particular disease type (centromere, nuclear dots)
  • Provides additional information for ANA -ve cytoplasmic antibodies (liver disease-endomysium, ANCA)

III. Patterns offer clue to which particular autoantibody may be present corresponding to particular disease type:

1. Homogenous – SLE

2. Speckled – Sjogrens Syndrome

3. Centromere – Scleroderma

4. Nucleolar – Scleroderma


IV. Confirmed by ANA Blot tests or monospecific ELISA
V. An excellent test for patients with:

  • Systemic Lupus Erythematosus (SLE)
  • Other connective tissue diseases

Clinical conditions confirmed by ANA BLOT

Target Antigen Clinical Association
SSA/ Ro Sjogren’s Syndome (SS), SLE/Neonatal Lupus
SSB/ La Sjogren’s Syndome ,SLE
Scl 70 PSS (Diffused)
PM- Scl Polymyositis (PM), Dermatomyositis (DM)
Proteins of inetochores (centromeres) CREST syndrome (PSS)
Jo-1 Polymyositis (PM)
Cyclin I (PCNA) SLE
Double strand DNA SLE
Nucleosomes SLE
Histones Drug Induced Lupus Erythematosus (LE)
Ribosome P phosphoprotein SLE
Mitochondrial (AMA- M2) Primary Biliary Cirrhosis (PBC)

Please book your appointment to take this test at our central processing lab.