{"id":2152,"date":"2024-12-06T04:35:12","date_gmt":"2024-12-06T04:35:12","guid":{"rendered":"https:\/\/suburban-blog.abymtech.com\/?p=2152"},"modified":"2025-03-05T10:12:34","modified_gmt":"2025-03-05T10:12:34","slug":"hla-b27-detection-with-flow-cytometry-and-pcr","status":"publish","type":"post","link":"https:\/\/www.suburbandiagnostics.com\/blog\/hla-b27-detection-with-flow-cytometry-and-pcr\/","title":{"rendered":"HLA B27 Detection: Flow Cytometry & PCR"},"content":{"rendered":"

\"\"<\/p>\n

HLA-B27 – CLASS 1 MHC protein is the hallmark of the genetic component of spondyloarthritides, and about 90% of patient with ankylosing spondylitis (AS) carry this antigen – it is\u00a0 present in 80-95% of patients with AS, in contrast to only 8% of the general population.<\/p>\n

Currently, there are 2 major methodologies available for the testing of HLA-B27:<\/p>\n

    \n
  1. Flow Cytometric Immunophenotypic (FCIPT)<\/li>\n
  2. Polymerase Chain Reaction (PCR)<\/li>\n<\/ol>\n\n\n\n\n\n
    FCIPT<\/strong><\/b><\/td>\n<\/tr>\n
    Pros<\/td>\nCons<\/td>\n<\/tr>\n
    \n
      \n
    • Cheaper than PCR<\/li>\n
    • Faster Turnaround time than PCR<\/li>\n<\/ul>\n<\/td>\n
    		A small percentage of cases give indeterminate\/inconclusive results which can be resolved only on testing with PCR.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n
    PCR<\/strong><\/b><\/td>\n<\/tr>\n
    Pros<\/td>\nCons<\/td>\n<\/tr>\n
    Definitive – no inconclusive\/indeterminate results, unlike flow cytometry.<\/td>\n\n