{"id":2151,"date":"2024-12-06T04:33:27","date_gmt":"2024-12-06T04:33:27","guid":{"rendered":"https:\/\/suburban-blog.abymtech.com\/?p=2151"},"modified":"2025-03-05T10:12:42","modified_gmt":"2025-03-05T10:12:42","slug":"biofire-filmarray-panel","status":"publish","type":"post","link":"https:\/\/www.suburbandiagnostics.com\/blog\/biofire-filmarray-panel\/","title":{"rendered":"Biofire FilmArray Panel Test to detect pathogens"},"content":{"rendered":"<p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone wp-image-2166\" src=\"https:\/\/suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-300x180.webp\" alt=\"\" width=\"1342\" height=\"805\" title=\"\" srcset=\"https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-300x180.webp 300w, https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-768x461.webp 768w, https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens.webp 1000w\" sizes=\"(max-width: 1342px) 100vw, 1342px\" \/><\/p>\n<p style=\"font-weight: 400;\">The Biofire FilmArray Panels perform multiplex testing to detect pathogens on specifically mentioned samples:<\/p>\n<table>\n<tbody>\n<tr>\n<td><b>Panel<\/b><\/td>\n<td><b>Sample Type<\/b><\/td>\n<td><b>Container<\/b><\/td>\n<td><b>Transportation Temperature<\/b><\/td>\n<td><b>Turnaround Time<\/b><\/td>\n<\/tr>\n<tr>\n<td>BioFire Blood Culture Identification 2 (BCID2) FilmArray Panel<\/td>\n<td>Blood, pus or body fluid<\/td>\n<td>Blood culture bottle (paired)<\/td>\n<td>Room temperature<\/td>\n<td>4 hours<\/p>\n<p>(post blood culture flagging positive)<\/td>\n<\/tr>\n<tr>\n<td>Gastrointestinal FilmArray Panel<\/td>\n<td>Stool<\/td>\n<td>Cary Blair transport medium\/ sterile container<\/td>\n<td>2\u00b0C &#8211; 8 \u00b0C (if transported in Cary Blair medium)<\/p>\n<p>Frozen (if Cary Blair medium is not available)<\/td>\n<td>4 hours<\/td>\n<\/tr>\n<tr>\n<td>Respiratory 2.1 FilmArray Panel<\/td>\n<td>Nasopharyngeal swab<\/td>\n<td>3ml of viral transport media (VTM) \/ saline<\/td>\n<td>At room temperature for up to 4 hours (15\u00b0C &#8211; 25 \u00b0C)<\/p>\n<p>Refrigerated for up to 3 days (2\u00b0C &#8211; 8 \u00b0C)<\/td>\n<td>4 hours<\/td>\n<\/tr>\n<tr>\n<td>Pneumonia Plus FilmArray Panel<\/td>\n<td>Bronchoalveolar lavage (BAL)<\/p>\n<p>sputum (induced and expectorated)<\/p>\n<p>endotracheal aspirate (ETA)<\/td>\n<td>Sterile container<\/td>\n<td>Refrigerated for up to 1 day (2\u00b0C &#8211; 8\u00b0C)<\/td>\n<td>4 hours<\/td>\n<\/tr>\n<tr>\n<td>Meningitis\/Encephalitis (ME) FilmArray Panel<\/td>\n<td>CSF<\/td>\n<td>Sterile container<\/td>\n<td>CSF in frozen condition or transported on ice within 24-30 hrs<\/td>\n<td>4 hours<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"font-weight: 400;\"><b>Quality Control: In built extraction &amp; PCR control in the lyophilized form in the pouches ensures the quality check at critical steps of extraction &amp; PCR for sample inhibitory effect.\u00a0<\/b><\/p>\n<p style=\"font-weight: 400;\"><b>Result terminology:<\/b><\/p>\n<ul style=\"font-weight: 400;\">\n<li><b><strong>Detected:<\/strong><\/b><br \/>\nWhen the result is shown as detected for a specific pathogen\/ resistance gene, it means:<\/p>\n<ul>\n<li>Controls were successfully passed, AND<\/li>\n<li>The specific genetic material for the pathogen\/ resistance gene was detected.<\/li>\n<\/ul>\n<\/li>\n<li><b><strong>Not Detected:<\/strong><\/b><br \/>\n\u200bWhen the result is shown as not detected for a specific pathogen\/ resistance gene, it means:<\/p>\n<ul>\n<li>Controls were successfully passed, AND<\/li>\n<li>The specific genetic material for the pathogen\/ resistance gene was not detected.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p style=\"font-weight: 400;\"><b>Interpretation of antimicrobial resistance genes in the pneumonia and BCID2 Biofire panels:<\/b><\/p>\n<table>\n<tbody>\n<tr>\n<td><b>Antimicrobial resistance gene<\/b><\/td>\n<td><b>Interpretation<\/b><\/td>\n<\/tr>\n<tr>\n<td>CTX-M<\/td>\n<td><b>Production of extended-spectrum \u03b2-lactamase (ESBL)<\/b>(1)<\/p>\n<p>Resistance to a wide range of commonly used \u03b2-lactam antibiotics including third generation cephalosporins (cefotaxime and ceftriaxone, but not generally against ceftazidime)<\/td>\n<\/tr>\n<tr>\n<td>IMP<\/td>\n<td><b>Carbapenemase production<\/b>(2)<\/p>\n<p>Resistance to carbapenems<\/td>\n<\/tr>\n<tr>\n<td>KPC<\/td>\n<td><b>Carbapenemase production<\/b>(3)<\/p>\n<p>Resistance to carbapenems<\/td>\n<\/tr>\n<tr>\n<td>MCR-1<\/td>\n<td><b>Colistin resistance<\/b>(4)<\/td>\n<\/tr>\n<tr>\n<td>MecA \/ MecC, MREJ<\/td>\n<td><b>Presence of MRSA<\/b>(5)<\/td>\n<\/tr>\n<tr>\n<td>NDM<\/td>\n<td><b>Carbapenemase production<\/b>(3)<\/p>\n<p>Resistance to carbapenems<\/td>\n<\/tr>\n<tr>\n<td>OXA-48<\/td>\n<td><b>Carbapenemase production<\/b>(3)<\/p>\n<p>Resistance to carbapenems<\/td>\n<\/tr>\n<tr>\n<td>VanA\/B<\/td>\n<td><b>Vancomycin resistance<\/b>(6)<\/td>\n<\/tr>\n<tr>\n<td>VIM<\/td>\n<td><b>Carbapenemase production<\/b>(3)<\/p>\n<p>Resistance to carbapenems<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"font-weight: 400;\">References:<\/p>\n<ol>\n<li style=\"font-weight: 400;\">\u00a0Rossolini GM, D\u2019Andrea MM, Mugnaioli C. The spread of CTX-M-type extended-spectrum \u03b2-lactamases. Clin Microbiol Infect. 2008 Jan;14:33\u201341.<\/li>\n<li style=\"font-weight: 400;\">\u00a0Lowe CF, Matic N, Champagne S, Romney MG, Leung V, Ritchie G. The Brief Case: IMP, the Uncommonly Common Carbapenemase. Burnham C-AD, editor. J Clin Microbiol [Internet]. 2020 Mar 25 [cited 2021 Dec 21];58(4). Available from: https:\/\/journals.asm.org\/doi\/10.1128\/JCM.01094-19<\/li>\n<li style=\"font-weight: 400;\">\u00a0Han R, Shi Q, Wu S, Yin D, Peng M, Dong D, et al. Dissemination of Carbapenemases (KPC, NDM, OXA-48, IMP, and VIM) Among Carbapenem-Resistant Enterobacteriaceae Isolated From Adult and Children Patients in China. Front Cell Infect Microbiol. 2020 Jul 3;10:314.<\/li>\n<li style=\"font-weight: 400;\">\u00a0Li B, Yin F, Zhao X, Guo Y, Wang W, Wang P, et al. Colistin Resistance Gene mcr-1 Mediates Cell Permeability and Resistance to Hydrophobic Antibiotics. Front Microbiol. 2020 Jan 10;10:3015.<\/li>\n<li style=\"font-weight: 400;\">\u00a0Becker K, Denis O, Roisin S, Mellmann A, Idelevich EA, Knaack D, et al. Detection of mecA and mecC Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay. Burnham C-AD, editor. J Clin Microbiol. 2016 Jan;54(1):180\u20134.<\/li>\n<li style=\"font-weight: 400;\">\u00a0Phukan C, Lahkar M, Ranotkar S, Saikia K. Emergence of vanA gene among vancomycin-resistant enterococci in a tertiary care hospital of North &#8211; East India. Indian J Med Res. 2016;143(3):357.<\/li>\n<\/ol>\n<p style=\"font-weight: 400;\">\n","protected":false},"excerpt":{"rendered":"<p>The Biofire FilmArray Panels perform multiplex testing to detect pathogens on specifically mentioned samples: Panel Sample Type Container Transportation Temperature Turnaround Time BioFire Blood Culture Identification 2 (BCID2) FilmArray Panel Blood, pus or body fluid Blood culture bottle (paired) Room temperature 4 hours (post blood culture flagging positive) Gastrointestinal FilmArray Panel Stool Cary Blair transport<span class=\"post-excerpt-end\">&hellip;<\/span><\/p>\n<p class=\"more-link\"><a href=\"https:\/\/www.suburbandiagnostics.com\/blog\/biofire-filmarray-panel\/\" class=\"themebutton\">Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":2166,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"class_list":["post-2151","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-for-doctors"],"blog_post_layout_featured_media_urls":{"thumbnail":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-150x150.webp",150,150,true],"full":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens.webp",1000,600,false]},"categories_names":{"5":{"name":"For Doctors","link":"https:\/\/www.suburbandiagnostics.com\/blog\/for-doctors\/"}},"tags_names":[],"comments_number":"0","rttpg_featured_image_url":{"full":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens.webp",1000,600,false],"landscape":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens.webp",1000,600,false],"portraits":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens.webp",1000,600,false],"thumbnail":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-150x150.webp",150,150,true],"medium":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-300x180.webp",300,180,true],"large":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens.webp",1000,600,false],"1536x1536":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens.webp",1000,600,false],"2048x2048":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens.webp",1000,600,false],"shuttle-column1-1\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-1000x380.webp",1000,380,true],"shuttle-column1-1\/4":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-1000x285.webp",1000,285,true],"shuttle-column2-1\/2":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-570x285.webp",570,285,true],"shuttle-column2-2\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-570x380.webp",570,380,true],"shuttle-column3-1\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-380x107.webp",380,107,true],"shuttle-column3-2\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-380x254.webp",380,254,true],"shuttle-column4-2\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/12\/Biofire-FilmArray-Panel-Test-to-detect-pathogens-285x190.webp",285,190,true]},"rttpg_author":{"display_name":"Suburban","author_link":"https:\/\/www.suburbandiagnostics.com\/blog\/author\/suburban\/"},"rttpg_comment":0,"rttpg_category":"<a href=\"https:\/\/www.suburbandiagnostics.com\/blog\/for-doctors\/\" rel=\"category tag\">For Doctors<\/a>","rttpg_excerpt":"The Biofire FilmArray Panels perform multiplex testing to detect pathogens on specifically mentioned samples: Panel Sample Type Container Transportation Temperature Turnaround Time BioFire Blood Culture Identification 2 (BCID2) FilmArray Panel Blood, pus or body fluid Blood culture bottle (paired) Room temperature 4 hours (post blood culture flagging positive) Gastrointestinal FilmArray Panel Stool Cary Blair transport&hellip;Read&hellip;","_links":{"self":[{"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/posts\/2151","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/comments?post=2151"}],"version-history":[{"count":3,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/posts\/2151\/revisions"}],"predecessor-version":[{"id":4315,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/posts\/2151\/revisions\/4315"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/media\/2166"}],"wp:attachment":[{"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/media?parent=2151"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/categories?post=2151"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/tags?post=2151"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}