![\"\"](\"https:\/\/suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-300x180.webp\" "\\"\\"")
<\/p>\n<\/p>\n
India has the 2nd highest sepsis death rate in South Asia, i.e. 213 per 1,00,000 people in India.<\/p>\n
Two critical factors impacting blood culture reports are:<\/b><\/p>\n
– DISINFECTION OF SKIN PRIOR TO COLLECTION=> Aseptic precautions + chlorhexidine in 70% isopropyl alcohol (AHD3000) => minimizes contamination and false positives<\/p>\n
– ADEQUATE VOLUME OF BLOOD => Paired blood cultures: 1 aerobic and 1 anaerobic blood or 2 aerobic culture bottles to be used => maximizes yield of pathogens<\/p>\n
Volume of blood required:<\/b> For an adult, the recommended volume of blood to be obtained per culture is 20 – 30 ml. It is ideal to send paired blood culture i.e. an aerobic and an anaerobic bottle \u2013 each having 10 – 12 ml of blood.<\/p>\n
Studies show that detection sensitivity goes up from 73.1% with a single bottle set (20 ml) to 89.7% with 2 bottle sets (40 ml) to 98.3% with 3 bottle sets (60 ml).<\/p>\n
Organism Profile: <\/b>To understand the profile of organisms isolated in blood culture, Suburban Diagnostics performed a study on blood cultures done over a period of 6 years.<\/p>\n
– We found that the total positivity rate is 15.1%.<\/p>\n
– The highest proportion of positive blood cultures were for people above the age of 60 years (p<0.001).<\/p>\n
The following is the bacteriological profile found in our study:<\/p>\n
– 38.5% were gram positive organisms<\/p>\n
– 61.5% were gram negative organisms<\/p>\n
The most common gram positive bacteria isolated were:<\/p>\n
– Staphylococcus aureus<\/p>\n
– Enterococcus spp<\/p>\n
Vancomycin & linezolid were the most effective against gram positive bacteria.<\/p>\n
– Cephalosporins: >61% resistant<\/p>\n
– Carbapenems: >60% resistant<\/p>\n
– Aminoglycosides: 20-55% resistant<\/p>\n
– Fluoroquinolones: 23-51% resistant<\/p>\n
– Penicillins: 50-80% resistant<\/p>\n
The most common gram negative bacteria isolated were:<\/p>\n
– Salmonella spp.<\/p>\n
– E. coli<\/p>\n
– Klebsiella spp.<\/p>\n
Gram negative bacteria showed the following antimicrobial resistance patterns:<\/p>\n
– Cephalosporins: >70% resistant<\/p>\n
– Carbapenems: >40% resistant<\/p>\n
– Aminoglycosides: 35-40% resistant<\/p>\n
– Fluoroquinolones: 65% resistant<\/p>\n
It is important to note that fluoroquinolones which are the first line drugs of choice in salmonella infections were found to be ineffective in >65% of cases. This should highlight the importance of antibiotic stewardship and the need to keep a close eye on the current efficacy of our first line drugs.<\/p>\n
The isolation of non-fermenters (Acinetobacter baumannii, Pseudomonas aeruginosa Burkholderia cepacia & Stenotrophomonas maltophilia) is also on the rise.<\/p>\n
We found that the overall incidence of non-fermenters increased by 2.3x in the past 6 years.<\/p>\n
Acinetobacter baumannii was the most resistant organism with >70% susceptibility to most of the antibiotics except colistin.<\/p>\n
Increased use of colistin has also led to the rise of microbes inherently resistant to colistin like Burkholderia cepacia & Stenotrophomonas maltophila.<\/p>\n
This increase in incidence of non-fermenters is concerning because:<\/p>\n
– They are primarily opportunistic pathogens in hospital-acquired infections.<\/p>\n
– They possess an intrinsic resistance to many antimicrobial agents.<\/p>\n
\u00a0We offer blood culture results as soon as possible to clinicians with preliminary identification reports issued in 24 hours to ensure rapid and accurate initiation of therapy.<\/p>\n
BREAKPOINT MIC are included in positive reports which helps in choosing an appropriate antibiotic for the patient (the higher the breakpoint to mic ratio, the more effective the antibiotic)<\/p>\n
For example, consider two antibiotics X & Y.<\/p>\n
X has a MIC of 0.5 and breakpoint 2 (Breakpoint\/MIC ratio = 4).<\/p>\n
Y has a MIC of 02 and breakpoint 16 (Breakpoint\/MIC ratio = 8).<\/p>\n
In this case, Y is the more favourable drug as it has a higher Breakpoint\/MIC ratio.<\/p>\n","protected":false},"excerpt":{"rendered":"
India has the 2nd highest sepsis death rate in South Asia, i.e. 213 per 1,00,000 people in India. Two critical factors impacting blood culture reports are: – DISINFECTION OF SKIN PRIOR TO COLLECTION=> Aseptic precautions + chlorhexidine in 70% isopropyl alcohol (AHD3000) => minimizes contamination and false positives – ADEQUATE VOLUME OF BLOOD => Paired…<\/span><\/p>\n Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":1290,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5,9],"tags":[],"class_list":["post-1283","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-for-doctors","category-recent-articles"],"blog_post_layout_featured_media_urls":{"thumbnail":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-150x150.webp",150,150,true],"full":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6.webp",1000,600,false]},"categories_names":{"5":{"name":"For Doctors","link":"https:\/\/www.suburbandiagnostics.com\/blog\/for-doctors\/"},"9":{"name":"Recent Articles","link":"https:\/\/www.suburbandiagnostics.com\/blog\/recent-articles\/"}},"tags_names":[],"comments_number":"0","rttpg_featured_image_url":{"full":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6.webp",1000,600,false],"landscape":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6.webp",1000,600,false],"portraits":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6.webp",1000,600,false],"thumbnail":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-150x150.webp",150,150,true],"medium":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-300x180.webp",300,180,true],"large":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6.webp",1000,600,false],"1536x1536":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6.webp",1000,600,false],"2048x2048":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6.webp",1000,600,false],"shuttle-column1-1\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-1000x380.webp",1000,380,true],"shuttle-column1-1\/4":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-1000x285.webp",1000,285,true],"shuttle-column2-1\/2":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-570x285.webp",570,285,true],"shuttle-column2-2\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-570x380.webp",570,380,true],"shuttle-column3-1\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-380x107.webp",380,107,true],"shuttle-column3-2\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-380x254.webp",380,254,true],"shuttle-column4-2\/3":["https:\/\/www.suburbandiagnostics.com\/blog\/wp-content\/uploads\/2024\/11\/5c051a2e-f268-48f3-b040-19b33afd8fa6-285x190.webp",285,190,true]},"rttpg_author":{"display_name":"Suburban","author_link":"https:\/\/www.suburbandiagnostics.com\/blog\/author\/suburban\/"},"rttpg_comment":0,"rttpg_category":"For Doctors<\/a> Recent Articles<\/a>","rttpg_excerpt":"India has the 2nd highest sepsis death rate in South Asia, i.e. 213 per 1,00,000 people in India. Two critical factors impacting blood culture reports are: – DISINFECTION OF SKIN PRIOR TO COLLECTION=> Aseptic precautions + chlorhexidine in 70% isopropyl alcohol (AHD3000) => minimizes contamination and false positives – ADEQUATE VOLUME OF BLOOD => Paired…Read…","_links":{"self":[{"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/posts\/1283","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/comments?post=1283"}],"version-history":[{"count":4,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/posts\/1283\/revisions"}],"predecessor-version":[{"id":4303,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/posts\/1283\/revisions\/4303"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/media\/1290"}],"wp:attachment":[{"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/media?parent=1283"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/categories?post=1283"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.suburbandiagnostics.com\/blog\/wp-json\/wp\/v2\/tags?post=1283"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}