Symptoms Speak Volumes!

Ankylosing Spondylitis has vague clinical manifestations and is often misdiagnosed or diagnosed late!

APPROXIMATELY 90% OF PATIENTS WITH ANKYLOSING SPONDYLITIS ARE HLA B27 POSITIVE
HLA B27 Detection by PCR is useful:
  • For confirmatory diagnosis of a suspended case of Ankylosing Spondylitis
  • For evaluating the risk of developing Ankylosing Spondylitis
  • In the diagnostics workup of Reiter’s Syndrome and Anterior Uveitis
Why PCR is a better method than flow Cytometry for HLA B 27 testing
Flow Cytometry – Indentifies HLA – B27 alleles based on cell morphologyPCR – identifies the Gene encoding the HLA – B27 allele
It provides False – Positive results.
1) It cannot distinguish between HLA – B27 subtypes. Hence, gives a positive result even for HLA – B27:06 and HLA – B27:09 which are not associated with Ankylosing Spondylitis
Provides True-negative results & True positive results.
1) Can distinguish between HLA – B27 subtypes
2) Provides Negative results of HLA – B27:06 and HLA – B27:09 which are not associated with Ankylosing Spondylitis
Provides intermediate / borderline results in 2 – 3% casesIntermediate / Borderline samples can be classified as positive or negative
Test name: HLA B27 PCR
Sample Type: EDTA whole blood
Schedule: Daily 6 am
TAT: Same day 6 pm
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